Back to article: Nutrient sensing and cAMP signaling in yeast: G-protein coupled receptor versus transceptor activation of PKA
FIGURE 2: Nitrogen activates PKA in a transceptor-dependent, cAMP-independent manner. (A) Activation of the PKA target trehalase in nitrogen-starved cells after addition of 5 mM L-citrulline. Strains: wild type (closed circles) and gap1Δ (open circles). (B) Donor/FRET levels for cAMP determination in vivo after addition of 5 mM L-citrulline to nitrogen-starved cells of the wild type strain. (C) Activation of the PKA target trehalase in nitrogen-starved cells of the wild type strain after addition of 5 mM (NH4)2SO4. Strains: wild type (closed circles) and mep2Δ (open circles). (D) Donor/FRET levels for cAMP determination in vivo after addition of 5 mM ammonium sulfate to nitrogen-starved cells of the wild type strain.