Back to article: Nutrient sensing and cAMP signaling in yeast: G-protein coupled receptor versus transceptor activation of PKA
FIGURE 3: Phosphate and sulfate activate PKA in a transceptor-dependent, cAMP-independent manner. (A) Activation of the PKA target trehalase in phosphate-starved cells of the wild type strain after addition of 10 mM KH2PO4. Strains: wild type (closed circles) and pho84Δ (open circles). (B) Donor/FRET levels for cAMP determination in vivo after addition of 10 mM KH2PO4 to phosphate-starved cells of the wild type strain. (C) Activation of the PKA target trehalase in sulfur-starved cells of the wild type strain after addition of 5 mM (NH4)2SO4. Strains: wild type (closed circles) and sul1Δ sul2Δ (open circles). (D) Donor/FRET levels for cAMP determination in vivo after addition of 5 mM (NH4)2SO4 to sulfate-starved cells of the wild type strain.