FIGURE 1: HAC1-mRNA splicing is compromised by aerobic agitation.(A) Procedures for culturing and ER-stress induction of S. cerevisiae cells under the aerobically shaken condition or under the static condition. ER-stress agents (DTT, tunicamycin, or ethanol) were added to the media 4-hr after culture start. (B) Optical density of cultures (wild-type BY4742 cells) was monitored under non-stressing conditions. (C-E) Wild-type BY4742 cells were cultured and were ER-stressed (or remained non-stressed) as shown in panel A, and were checked for HAC1-mRNA splicing. n.s. (not significant): p > 0.05, *: p < 0.05, **: p < 0.01, ***: p < 0.001.