FIGURE 4: DadY is not required for catabolism of alanine by P. aeruginosa. P. aeruginosa wild-type (circles), dadA mutant (squares), and dadY mutant (triangles) strains were inoculated into minimal media with the carbon and nitrogen sources as indicated (carbon, nitrogen); (A) glucose, ammonia, (B) glucose, alanine, and (C) alanine, ammonia. D-alanine (closed shapes) or L-alanine (open shapes) was added as indicated as the source of carbon or nitrogen at 20 mM or 5 mM, respectively. When present glucose was at 11 mM and ammonia was at 10 mM. Growth was measured as the change in optical density at 650 nm over time. Error bars represent standard deviation between three independent biological replicates.