FIGURE 2: Effects of cholesterol, U18666A and ketoconazole on bacterial growth and luminescence production in axenic cultures. (a-c) Luminescence output of the Lp icmRp–LuxR measured every ten min in axenically grown AYE liquid cultures in the presence/absence of different concentration of cholesterol (a), U18666A (b), or ketoconazole (c) over the indicated time period. Each timepoint represents an average of technical triplicates. Grey panel denotes entry into stationary phase. (a-c) Representative data from one of three biological replicates are shown.