FIGURE 5: Different wild type backgrounds considerably differ in their targeting competence. Cells of YPH499 and BY4742 wild types were transformed with NeonGreen-expressing plasmids differing in promoter strength and peroxisomal targeting sequences. Cells were grown in glucose or lactate medium for the times indicated. Whereas peroxisomal targeting worked well in the BY4742 cells, the fusion proteins showed a cytosolic distribution in the YPH499 background. However, the carbon source used had no influence on the targeting, but on the number of peroxisomes per cell. Scale bar, 5 µm.