Back to article: GFP fusions of Sec-routed extracellular proteins in Staphylococcus aureus reveal surface-associated coagulase in biofilms
FIGURE 1: Visual schematics of constructs expressing fusion proteins under control of the inducible Pxyl/tetO promoter in pRMC2. (A) Tat:msfGFP, (B) Sec:msfGFP, and (C) msfGFP control. SD = Shine-Dalgarno sequence, SP = signal peptide, and L = linker. Amino acid sequences for the Tat signal peptide [18], Sec signal peptide [45] and linker are given in the figure. DNA sequences are provided in Supplementary S1.
18. Biswas L, Biswas R, Nerz C, Ohlsen K, Schlag M, Schäfer T, Lamkemeyer T, Ziebandt AK, Hantke K, Rosenstein R, Götz F (2009). Role of the twin-arginine translocation pathway in Staphylococcus. J Bacteriol 191(19): 5921–5929. 10.1128/JB.00642-09
45. Schallenberger MA, Niessen S, Shao C, Fowler BJ, and Romesberg FE (2012). Type I signal peptidase and protein secretion in Staphylococcus aureus. J Bacteriol 194(10): 2677–2686. 10.1128/JB.00064-12