FIGURE 7: Tim9, but not Dre2, is oxidized by recombinant Mia40 in vitro.

Radiolabeled imsDre2, Dre2 and Tim9 were reduced and either directly loaded to the gel (unmodified, unmod.), or incubated with 35 µM Mia40^SPS or Mia40 for the times indicated, denatured with SDS and treated with 15 mM mPEG-12. For control, a sample of reduced protein was directly treated with mPEG-12 so that all thiols are alkylated (maximum shift, max.).