FIGURE 5: CWI mutants are defective in acetic acid-induced cytochrome c release.

Western blot analysis of cytochrome c in S. cerevisiae strains BY4741, bck1Δ, slt2Δ and rlm1Δ before (-) and after (+) exposure to 120 mM acetic acid, pH 3.0, for 200 min, in both mitochondrial and cytosolic fractions. Cytosolic phosphoglycerate kinase (Pgk1p) and mitochondrial porin (Por1p) levels were used as loading control of cytosolic and mitochondrial fractions, respectively. A representative experiment is shown of at least two independent experiments with similar results.