FIGURE 2: Immunofluorescence analysis of U-2 OS cells expressing a TcoClec protein. U-2 OS cells expressing a TcoClec protein are indicated (white arrowheads).

(A) Cells were stained with anti-TcoRep, anti-calnexin and DAPI. Merged picture (bottom right) shows that TcoClec and calnexin colocalize partially.

(B) Three patterns could be observed in the same proportions: endo-plasmic reticulum (ER, left), plasma membrane (PM, middle) and both localizations (right). Cells were stained with anti-TcoRep and DAPI. Bar, 20 µm.