FIGURE 1: Proline/glutamate metabolism as the sole carbon source and its control mediated by Fmp12.

(A) Growth phenotypes of S. cerevisiae wild-type strain (BY4741) and its deletion mutants on SD, SD-N-C+Pro, SD-N-C+Glu, and SD-N+Pro media.

(B) Growth phenotypes of S. cerevisiae BY4741u or BY4741u Δfmp12 cells harboring the empty vector (pVV208, pVV209) or overexpressing FMP12 (pVV208-FMP12, pVV209-FMP12) on SD, SD-N-C+Pro, and SD-N-C+Glu media.

(C) Detection of Fmp12-3HA in the whole cell extract (WCE) and in the mitochondria fraction (Mt). The symbol -/+ indicates the sample from the cells without or with expression of Fmp12-3HA from pVV209-FMP12. The arrow indicates the predicted size of Fmp12-3HA truncated at its amino-terminal MTS. GAPDH and porin were analyzed as controls of cytoplasmic and mitochondrial proteins, respectively.

(D) Subcellular localization of Fmp12-yeGFP by fluorescent microscopy. GFP signals (GFP), MitoTracker signals (Mt), merged signals (Merged), and differential interference contrast images (DIC) are shown. The scale bar represents 5 μm.

(E) Western blot analysis of Fmp12-yeGFP under different nutritional conditions. S. cerevisiae FMP12-yeGFP cells were cultivated on solid media at 30°C for 5 days. The numbers indicate the media as follows: 1: SD, 2: SD+Pro, 3: SD-N-C+Pro, 4: SD-C+Pro, 5: SD-N-C+Glu, 6: SD-C+Glu. GAPDH was used as a protein-loading control.

(F) Energy production by proline metabolism as the sole carbon source in S. cerevisiae. Pro: proline, P5C: Δ1-pyrroline-5-carboxylate, Glu: glutamate, α-KG: α-ketoglutarate, Suc-CoA: succinyl-CoA, Suc: succinate.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.

Close