FIGURE 2: S. cerevisiae (rho⁺) cells co-expressing Abp1-RFP and Abp140-GFP from chromosomal sites (strain CRY1337). They were inspected after fixation with 3.7 % formaldehyde for 30 minutes (HCHO-fixed) (A) or as live cells (B). (Glu+) glucose was present in the medium; (Glu-) cells were shifted to glucose-free medium and cultivated for an additional 30 or 80 minutes before fixation or inspection. Distribution of fluorescent markers is presented after deconvolution and projection of several images in the stack (Z-stacks) using Xcellence software (Olympus). Bar, 5 µm.