FIGURE 4: Quantification of CSP expression in modified Entamoeba histolytica trophozoites.

Five, ten and twenty µg of protein from crude trophozoite extracts were loaded. CSP (140 kDa) and actinin (70 kDa) were revealed with specific antibodies. A decrease in CSP abundance was observed in trophozoites expressing the CSP-AS construct. Amounts of CSP and actinin in 20 µg of crude extract were quantified using AlphaEaseFC software. Levels of CSP were normalized against the amount of α-actinin. The relative abundance of CSP was 57% lower in CSP-AS trophozoites than in WT and GFP trophozoites (P = 0.04). Data are representative of three independent experiments.