FIGURE 5: Despite its location in the IMS, imsDre2 does not contain disulfides.

(A, B) The redox state of disulfides in imsDre2 and control proteins was analyzed in wild type, ∆por1 and ∆glr1 cells as described in Fig. 3.

(C) Mitochondria were isolated from imsDre2-expressing cells, incubated in the presence or absence of diamide or TCEP. Proteins were TCA-precipitated before their redox state was analyzed as described before.