FIGURE 6: A large proportion of NQ cells have one or no bud scar. After the bud scars of Q and NQ cells were stained with calcofluor white, z-stack images were taken and analyzed manually. Three repeats were performed for each cell type and at least 100 cells were analyzed in each repeat. Error bars represent the standard error. The left panel shows typical images of cells with 0, 1, 2 and 3 bud scars.