FIGURE 3: Toxicity of DMB and analogs. Wild-type S. Typhimurium was grown in NCE minimal medium supplemented with glycerol, succinate or tricarballylate as the main source of carbon and energy as described in Materials and Methods. Different bases were supplemented at 1 or 4 mM final concentration as indicated. Error bars represent standard deviation of three technical replicates and each experiment was completed with three biological replicates, with a representative graph shown. Error bars smaller than the symbols are not shown. Abbreviations: DMPDA, 4,5-dimethyl-1,2-phenylenediamine; 5-OH-Bza, 5-hydroxybenzimidazole; Bza, benzimidazole; 5-MeO-Bza, 5-methoxybenzimidazole; 5-Me-Bza, 5-methylbenzimidazole; DMB, 5,6-dimethylbenzimidazole. Structural differences of these bases in comparison to DMB are shaded in blue.