FIGURE 8: Overexpression of tcuC is sufficient for DMB resistance in tricarballylate. (A) relative genetic context depicting gain of function mutation in strain JE26839. This strain was originally isolated from tricarballylate minimal medium contained DMB (1 mM). Tricarballylate utilizing genes are arranged into an operon in Salmonella, these genes include tcuA encoding tricarballylate dehydrogenase, tcuB encoding FADH2; quinone oxidoreductase and tcuC encodes for tricarballylate transporter [51]. (B) Growth analysis to study DMB resistance in tricarballylate is shown. Cells were grown in NCE minimal medium supplemented with tricarballylate (20 mM), glucose (0.5 mM) and DMB (1 mM). l-(+)-Arabinose was used at a 0.5 mM final concentration for induction. DMB resistant mutant represented here is JE26839. Error bars represent standard deviation of three technical replicates and each experiment was completed with three biological replicates, with a representative graph shown.