FIGURE 3: Caspase 3 inhibits TDP43 protein aggregation in skeletal muscle cells. (A) Representative immunofluorescence image of C2C12 cells treated with DMSO, z-DEVD-FMK and PAC1 showing protein PHB2 (green) and TDP43 (red) and their colocalization (yellow). The graph represents the percentage of TDP43 inclusions in C2C12 cells treated with DMSO, z-DEVD-FMK, and PAC1. The experiments were performed in triplicates and the values are represented as mean ± SEM with p-value < 0.05. (B) Representative immunofluorescence image of human primary myoblast treated with DMSO and PAC1 showing protein PHB2 (green) and TDP43 (red) and their colocalization (yellow). The graph represents the percentage of TDP43 inclusions in n primary myoblast cells treated with DMSO, z-DEVD-FMK, and PAC1. The experiments were performed in triplicates and the values are represented as mean ± SEM with p-value < 0.05 (C) Representative fluorescence image of C2C12 cells treated with DMSO and z-DEVD-FMK stained with MitoTracker Red CMXRos for assessment of mitochondrial function. The graph represents difference in mean fluorescence intensity of MitoTracker Red CMXRos (red) in C2C12 cells treated with DMSO and z-DEVD-FMK. The experiments were performed in triplicate with values represented as mean ± SEM with p-value < 0.05.