FIGURE 3: Deleting aif1 in the acetic acid-sensitive mutants increases their survival to different degrees in acetic acid. (A) aif1Δ produced in the wild-type (H3 WT), H3S28A or H3Q68A were spotted in YPD pH 4.5 only (untreated) or different concentrations of acetic acid (30, 40, 60 mM). Cells were grown at 30 °C for 72h and scanned. (B) the cells used in panel A were grown in liquid culture under untreated (UT) or 30 mM acetic acid treated (AA) conditions. The graphs reflect the absorbance values at 600 nm at every 30' for the hours indicated in the x-axis.