FIGURE 8: Acetic acid-sensitive mutants exhibit sensitivity towards disruption of ER homeostasis. (A) Spot-test assays of the wild-type (H3 WT) and mutants in the presence of different concentrations of tunicamycin (Tm) (0.5 μg/mL, 0.75 μg/mL). (B) Spot-test assays of the indicated strains in YPD only (untreated) or Congo red (CR) (100 μg/mL) or B, Caffeine (Caf) (10 mM). The spotted cells were grown at 30°C for 48h (CR) or 72h (Caf) and scanned. (C) Spot-test assays of the indicated strains in YPD supplemented with DMSO, which serves as control (DMSO) or different concentrations of tunicamycin (Tm) (0.25 μg/mL, 0.5 μg/mL). Note: C1 and C2 refer to clones 1 and 2, respectively.