Back to article: Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways


FIGURE 20: Microscopy-based assay for quantitative measurement of DSB end resection rates in single cells. (A) Cartoon diagram of the assay principles, including a recognition site for the HO nuclease adjacent to a LacO array. Resection drives loss of LacI-GFP and binding of Rad52-mCherry. On the right are examples of images that highlight the phases of DSB induction and processing. (B) Time-lapse movie of two sister cells that each form a DSB in S phase and undergo resection. The first frame when Rad52-mCherry is detected at the DSB and last frame in which the LacI-GFP focus is detected are boxed in blue. This gives rise to the resection duration, from which the resection rate can be calculated (blue). The time intervals are 10 minutes.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.

Close