Back to article:
Type II-Metacaspases are involved in cell stress but not in cell death in the unicellular green alga Dunaliella tertiolecta
FIGURE 5. MCs and CL enzymatic activities in D. tertiolecta exposed to PAR + UVA + UVB (PAB) for six days. Michaelis-Menten kinetics showed by Boc-7-amino-4-fluoromethyl coumarin (AMC) reporters of the substrates RVRR (Δ), QRR (•), GRR (○), LKR (▾), HEK (▪), and VLK (□) (A); Michaelis-Menten kinetics showed by Ac-7-amino-4-trifluoromethyl coumarin (AFC) reporters of the substrates WEHD (•), DEVD (○), IETD (▾), and LEHD (Δ) (B); Cleavage activity showed by D. tertiolecta under PAB of (AMC) reporters of the substrates RVRR (Δ), QRR (•), GRR (○), LKR (▾), HEK (▪), and VLK (□) (C); Cleavage activity showed by D. tertiolecta under PAB of (AFC) reporters of the substrates WEHD (•), DEVD (○), IETD (▾), and LETD (Δ) (D); Cleavage activity showed by D. tertiolecta during dark-induced CD of (AMC) reporters of the substrates with R in P1 (E), symbols as in (A). Symbols are means of measurements of two independent replicate cultures and three replicate samples from each culture cylinder. Error bars indicate standard deviations.