Back to article: Depletion of SNAP-23 and Syntaxin 4 alters lipid droplet homeostasis during Chlamydia infection


FIGURE 3: Depletion of SNAP-23 and Syntaxin 4 increases LD content during Chlamydia infection without affecting LD internalization into the inclusion lumen. (A) Experimental design. SN23 = SNAP-23, Syn3 = Syntaxin 3, Syn4 = Syntaxin 4, LDs = lipid droplets, MOI = multiplicity of infection. (B and C) Cells were either infected with mCherry-expressing C. trachomatis L2 at a MOI of 1 or mock-infected with DMEM for 24 h. The cells were then stained with BODIPY and fixed. BODIPY intensity was measured by flow cytometry. For the infected samples only the mCherry-positive population (infected cells) was measured. (B) Graph represents the average percent increase in BODIPY staining relative to non-infected cells from at least three independent experiments ± the standard deviation. Asterisk (***) denotes a p value < 0.001. (C) Graph represents the average increase in BODIPY staining relative to infected empty vector control cells from at least three independent experiments ± the standard deviation. The percent increase in BODIPY staining following infection for the empty vector control cell line was arbitrarily set at 100% and represents 121.41% ± 4.92. Values for the KD cells were then normalized to the EV control. Asterisk (***) denotes a p value < 0.001 and (****) denotes a p value <0.0001. ns = not significant. (D) The indicated cell lines were infected with C. trachomatis L2 at a MOI of 0.5 for 30 h. The cells were then fixed and stained with anti-IncA antibody (red) and BODIPY 493/503 (green). Hoechst was used to label DNA (gray). Images are taken from a single plane of confocal z-stacks. Orthogonal XZ and YZ views are shown next to and below the overlay image, respectively. Scale bar = 20 μm. KD = knock down.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.

Close