FIGURE 3: OPLS-DA of amino acid analysis data from yeast mutants involved in proline metabolism. (A) Score plot of OPLS-DA for the wild-type strain (WT; BY4741) and short- and long-lived mutants involved in proline metabolism. (B) Loading plot for DA1 in panel (A). (C) Each point represents an individual batch from WT and the mutants for indicated gene at log phase. The values are the means and standard deviation of results from three independent experiments. *, p < 0.05; **, p < 0.01, versus WT calculated with the Dunnett's test.