FIGURE 5: The assembly of PulD-T in mitochondria is enhanced in the absence of Tom20. (A) Mitochondria were isolated from the indicated strains transformed with either an empty vector (Ø) or a plasmid encoding PulD-T. Further treatment and analysis were as described in the legend of Fig. 4A. (B) Crude mitochondria were obtained from wild type (WT), tom20Δ or a strain overexpressing TOM20 (Tom20↑) harbouring a plasmid encoding PulD-T. Further treatment and analysis were as described in the legend of Fig. 4B. (C) Crude mitochondria were obtained from WT, tom70/71Δ or a strain overexpressing TOM70 (Tom70↑) harbouring a plasmid encoding PulD-T. Further treatment and analysis were as described in the legend of Fig. 4B.