FIGURE 7: Lack of Tom70 improves the biogenesis of SsaC in mitochondria. (A) Isolated mitochondria were obtained from the indicated strains transformed with either an empty vector (Ø) or a plasmid encoding SsaC-HA. Further treatment and analysis were dones as described in the legend of Fig. 4A. (B) Crude mitochondria were obtained from widll type (WT), tom70/71Δ or a strain overexpressing TOM70 (Tom70↑) harbouring a plasmid encoding SsaC-HA. Further treatment and analysis were done as described in the legend of Fig. 4B. (C) Whole cell lysate was obtained from the indicated cells and was analysed by SDS-PAGE followed by immunodecoration with antibodies against the indicated proteins. The steady state levels of SsaC-HA in at least three experiments for each strain were quantified and further analysis was as described in the legend of Fig. 6D. (*, P < 0.05; two tailed Student's t-test).