FIGURE 1: Effects of macrophage iron loading on mRNA expression of metabolic enzymes. Expression of aconitase (A), isocitrate dehydrogenase (B), succinate dehydrogenase (C) and lactate dehydrogenase (D) mRNA relative to the house keeping gene was determined by qRT-PCR after treating RAW264.7 cells with either FeCl₃ (50 μM), hepcidin (1 μg/ml) or DFP (50 μM) for 30 hours. Representative data from two independent experiments performed with 5 and 6 replicates are shown. Graphs show means ± SEM. One-way ANOVA with Tukey's multiple comparison correction was performed. Exact p-values are indicated in the graphs.