FIGURE 2: Effects of Salmonella infection on the expression of metabolic enzymes and metabolites in RAW264.7 macrophages. Cells were infected with Salmonella enterica serovar Thyphimurium (MOI of 10) as detailed in methods. Expression of aconitase (A), isocitrate dehydrogenase (B), succinate dehydrogenase (C) and lactate dehydrogenase (D) mRNA relative to the house keeping gene was determined by qRT-PCR in untreated control cells (ctrl) or infected cells after 24 hours. Pyruvate, lactate and methionine sulfoxide levels (E-G) were determined in cell culture supernatants. Representative data from two independent experiments performed with 3 and 6 replicates are shown. Graphs show means ± SEM. Student's t-test was used. Exact p-values are indicated in the graphs.