FIGURE 4: Infection but not iron perturbations induces mTOR activation in macrophages. Protein levels of the mTOR downstream target p4E-BP1 were determined using Western blot analysis after treating RAW264.7 cells with Salmonella (MOI of 10) for 1 h. Additionally, cells were treated with FeCl3/hepcidin (50 μM/1 μg/ml, respectively) or DFP (50 μM) for 6 h prior to infection.