FIGURE 6: Effect of Salmonella infection, iron perturbation and mTOR inhibition on iron homeostasis in RAW264.7 cells. Protein levels of transferrin receptor (TfR) and ferritin were determined in control and Salmonella infected RAW264.7 cells after 24 hours of infection and either pretreatment with FeCl₃/hepcidin or DFP and/or rapamycin for 6 or 1 hours respectively. Representative blots (A) and densitometric quantification of results relative to beta-actin expression (B) are shown. Graphs show means ± SEM. One-way ANOVA with Tukey's multiple comparison correction was performed. Exact p-values (< 0.05) are indicated in the graphs.