Back to article: Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes

FIGURE 3: MutS localizations and co-localizations in B. subtilis cells. (A) Photoactivated localization microscopy (PALM) reconstruction (left; magenta) and single-molecule trajectories (right; red) of MutS-PAmCherry overlaid with DnaX-mCitrine (green and blue) and phase-contrast cell images. Overlapping signals are in white and orange arrows indicate replisome regions at which MutS enrichment is observed. (Scale bar: 1 μm). (B) Localization probability density maps of DnaX-mCitrine (upper; blue-green) and MutS-PAmCherry (lower; red-yellow) within a normalized cell. (C) Normalized cell maps as in (B) but for the MutS[F30A] mutant shows that the localization of MutS to the replisome is independent of the ability of MutS to identify mismatches. Reproduced from Liao et al. PNAS 2015 [41].

41. Liao Y, Schroeder JW, Gao B, Simmons LA, Biteen JS (2015). Single-molecule motions and interactions in live cells reveal target search dynamics in mismatch repair. Proc Natl Acad Sci U S A 112(50): E6898-E6906. 10.1073/pnas.1507386112

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