Back to article: Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes

FIGURE 9: Effect of Pif1 DNA helicase on Pol δ-mediated DNA synthesis. (A) Reaction scheme to examine the effect of S. cerevisiae Pif1 helicase on Pol δ-mediated DNA synthesis. (B) Reactions conducted with PCNA-RFC-Pol δ in conjunction with Pif1 or the helicase-dead mutant pif1 K264A (K/A). Analysis was by (i) native gel electrophoresis and (ii) denaturing gel electrophoresis (7M urea, polyacrylamide) [114]. The asterisk identifies extended invading ssDNA species that had been released from the D-loop by Pif1. (The gel image is taken from [114]).

114. Wilson MA, Kwon Y, Xu Y, Chung WH, Chi P, Niu H, Mayle R, Chen X, Malkova A, Sung P, Ira G (2013). Pif1 helicase and Poldelta promote recombination-coupled DNA synthesis via bubble migration. Nature 502(7471): 393-396. 10.1038/nature12585

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