Back to article: Variants of the human RAD52 gene confer defects in ionizing radiation resistance and homologous recombination repair in budding yeast

FIGURE 2: The adh1::HsRAD52-G59R-FLAG and adh1::HsRAD52-S346X-FLAG alleles fail to suppress the ionizing radiation sensitivity of rad52-/- mutant yeast cells. Cultures of wild-type (ABX3566), rad52-/- (ABX3568), rad52-/- adh1::HsRAD52-FLAG+/+ (ABX4130), rad52-/- adh1::HsRAD52-G59R-FLAG-/- (ABX4129) and rad52-/- adh1::HsRAD52-S346X-FLAG-/- (ABX4131) yeast strains grown to mid-log phase were counted before being subjected to 20, 40, 80, 160 and 320 Gy of X-ray radiation. Appropriate dilutions of unirradiated and irradiated cultures were plated onto solid YPD medium, incubated at 30°C for three days, and the resulting colonies counted. Percent viability was calculated by dividing the number of colonies arising on the plates by the number of cell bodies plated and multiplying by 100. Mean percent survival was calculated for at least 10 independent cultures for each genotype. These values and 95% confidence intervals were plotted against levels of radiation exposure.

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