FIGURE 3: GFP^PEST as a reporter for transient changes in CN activity.(A-D) Flow cytometric quantification of GFP intensities (A, C) and corresponding histograms of selected time points (B, D) after addition of cycloheximide (CHX) to wild type cells pre-treated with 50 mM Ca²⁺ for 1 h (A, B) or to Δpmr1 cells (C, D) equipped with either pAMS366-4xCDRE-GFP or pAMS366-4xCDRE-GFP^PEST. (E-H) Flow cytometric quantification of GFP intensities (E, G) and corresponding histograms of selected time points (F, H) after addition of 0.5 µM FK506 to wild type cells pre-treated with 50 mM Ca²⁺ for 1 h (E, F) or to Δpmr1 cells (G, H) equipped with pAMS366-4xCDRE-GFP^PEST. Dead cells were excluded from the analysis via propidium iodide (PI) staining. Means ± SEM, n = 4.