FIGURE 3: Hyper-recombination phenotype of H3E73Q and H4E53A mutants is not associated with defective transcription.(A) A scheme of the direct-repeat recombination system with different promoters is shown. GAL1 promoter (GAL1p) is induced in 2% galactose (ON) and repressed in 2% glucose (OFF). Transcription from CLB2 promoter (CLB2p) is restricted to G2 phase. Transcription from HHF2 promoter (HHF2p) is restricted to S phase. (B) Analysis of direct-repeat recombination frequencies in H3 wild-type (H3WT) and H3E73Q (H3E73Q) strains transformed with pARSLlacZGLB-in, pARSLlacZHLB-IN or pARSLlacZBLB plasmids with the direct-repeat recombination system under GAL1, HHF2, CLB2 promoter respectively (n=3). (C) Analysis of direct-repeat recombination frequencies in H4 wild-type (H4WT) and H4E53A (H4E53A) strains transformed with pARSLlacZGLB-in, pARSLlacZHLB-IN or pARSLlacZBLB plasmids with the direct-repeat recombination system under GAL1, HHF2, CLB2 promoters, respectively (n=3). Means and SEM are plotted in (B) and (C). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 (two-tailed Student's t-test).