FIGURE 3: Multiple alignment of the amino acid sequences of the studied UPF0016 members. The alignment was carried out using Clustal Omega and visualized in Jalview. Residues are shaded in blue depending on their conservation. The conservation histogram provides quantitative evaluation of the degree of conservation of the physico-chemical properties of the amino acids at the corresponding position. Putative transmembrane spans predicted by TMAP are indicated above the sequences (TM1 – TM6). The highly conserved UPF0016 motifs are framed in orange. Residues identified as altering the protein stability, localization, and/or activity are highlighted in red.