FIGURE 1: Impact of CRISPR/Cas9 technology on the generation of null mutants in Trypanosoma cruzi. Linear plots show the cumulative number of endogenous genes for which ablation attempts have been published per year in T. cruzi, using conventional knockout (blue), CRISPR/Cas9 (red), and the total number using either of the two methods (black). Only the first attempts reported for each gene were considered in the analysis.