Back to article: Aeration mitigates endoplasmic reticulum stress in Saccharomyces cerevisiae even without mitochondrial respiration

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FIGURE 2: Hypoxic treatment boosts HAC1-mRNA splicing even in agitated cultures.(A) Procedures for culturing and ER-stress induction of S. cerevisiae cells under the aerobically stirred condition or under the nitrogen-gas filled condition. DTT was added to the media 4 hr after culture start. (B) Optical density of cultures (BY4742 cells) was monitored under non-stress conditions. (C) BY4742 cells were cultured and were ER-stressed (or remained non-stressed) as shown in panel A, and were checked for HAC1-mRNA splicing. n.s. (not significant): p > 0.05, **: p < 0.01.

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