Back to article: Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast

FIGURE 2: Long- and short-lived mutants and their functional enrichments.(A) Volcano plot of differences in mutant abundance on Day 3 relative to Day 0 (log2 fold-change), based on Bar-seq of re-growth experiment, using edgeR analysis of three independent repeats [86]. Significance was determined using a fold-change (FC) cut-off of |log2(FC)| >log2(1.5) and a false discovery rate (FDR) cut-off of <0.05. (B) Selected functional enrichments from Metascape [87] are shown for long- and short-lived mutants, including chromatin-related terms (green), autophagy-related terms (red), mitochondrial-related terms (blue) and other terms (black). The colour scale indicates significance expressed as -log10 p-values, and the size of the dots reflects the percentage of the input genes among all genes associated with the respective GO term.

86. Robinson MD, Mccarthy DJ, and Smyth GK (2010). edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26(1): 139–140. 10.1093/bioinformatics/btp616

87. Zhou Y, Zhou B, Pache L, Chang M, Khodabakhshi AH, Tanaseichuk O, Benner C, and Chanda SK (2019). Metascape provides a biologist-oriented resource for the analysis of systems-level datasets. Nat Commun 10(1): 1523. 10.1038/s41467-019-09234-6

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