FIGURE 1 (A, B) Flow cytometry analysis of exponential cells (overnight culture) prior to (A), and 14 days after (B) desiccation. Cells were fixed with 70% ice cold ethanol, stained with propidium iodide (PI) Cell Cycle solution, and analyzed using an Bio-Rad S3e cell sorter. Arrow indicates the small population of cells that had higher DNA content. 1n and 2n indicate one copy and two copies of DNA, respectively. (C) Percentage of live/dead cells in different cell cycle phases of exponential cells after 14 days of desiccation. Live and dead cells were identified by PI staining. Cell cycle status was determined by images of bright field and fluorescence brightener-28 stained cells. Approximately 400 cells were counted from each experiment and the data were presented as mean ± standard error from three independent experiments.