FIGURE 5: Confocal microscopy images of fluorescence stained log-phase cells after 14 days of desiccation, showing replicatively aged cells were more sensitive to desiccation. DRAQ5 stains nucleus, propidium iodide (PI) stains dead cells, fluorescence brightener 28 (FB) stains cell wall and bud scars, bright field (BF) shows the overall structure of the cell. Scale bar = 5 μm.