FIGURE 2: Deletion of PIF1 does not rescue telomere capping-deficient tlc1-tm cells. (A) Tenfold serial dilutions of strains with the indicated genotypes were spotted on YPD plates and grown at 25ºC or 30ºC. (B) A cdc13-1/CDC13 tlc1-tm/TLC1 diploid strain was sporulated and the resulting tetrads were dissected on YPD plates, which were incubated at 22ºC or 25ºC. Each column of colonies arose from a single tetrad.