FIGURE 5: Changes in protein expression levels in ISG75 knockout cells. (A) Volcano plot showing distribution of protein level changes in in ISG75Crc4 cells. Proteins and protein groups increased 1.2-fold, 1.5-fold to 2-fold and 2-fold or more with p-values < 0.05 are shown respectively in light blue, turquoise and dark blue filled circles, protein groups decreased 2-fold or more – in red, non-significantly shifting protein groups – in grey unfilled circles. (B) FGSEA output showing most enriched GO-terms among all proteins in A. Top five groups are shown in orange. Unfilled circles all proteins, filled circles proteins with increased expression (Table 1 and 2). Labelled circles with coloured outlines are proteins with most increased expression in these groups. (C) Parasitaemia levels in Balb/C mice blood on days three and four post infection with 10⁴ ISG75Crc4 cells and days four to six post infection with 10² cells, top and bottom respectively. Error bars, standard error. (D) Western Blot analysis of lysates of 2T1 and ISG75Crc4 cells extracted on day four terminal bleeds of mice infected with 10⁴ cells shows persistent depletion of ISG75 in trypanosomal cell when in host environment – similar to that observed in vitro.