FIGURE 2: A modular cloning workflow with sequence cassettes for intracellular protein targeting. (A) The defined syntax of the MoClo workflow allows the combination of sequence parts into expression plasmids. (B) Overview of the targeting sequences used in this study. The type designates the part according to the nomenclature used in a previous study [14]. TA, tail anchor of Ubc6. SS, signal sequence. OMM, outer mitochondrial membrane. See Materials and Methods for details.

14. Lee ME, DeLoache WC, Cervantes B, Dueber JE (2015). A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth Biol 4(9): 975-986. 10.1021/sb500366v