FIGURE 2: Snf1 localization and Snf1 phosphorylation. (A) The nuclear-cytosolic ratio of Snf1-GFP was calculated by dividing the mean of the fluorescence of Snf1-GFP in the nucleus with the mean of the fluorescence in the cytosol. Horizontal lines indicate the mean, the boxplot has as lower and upper hinge respectively the 25^thand 75^th percentile and the whiskers denote the 95% confidence interval. Dots denote individual cells. (B) Mean of relative Snf1 phosphorylation by western blot quantification 5 minutes after upshift from ethanol to glucose, fructose or mannose, dots represent biological replicates.(C) Exemplary images of the western blot showing phosphorylated Snf1.