FIGURE 4: A truncated version of Rrp6p consisting of a deletion of the C-terminal domain that abolishes its interaction with the core exosome is equally competent in maintaining the steady-state levels of the polyadenylated version of mature populations of all sncRNAs like its native full-length counterpart. (A) Cartoon showing the various functional domains of native full-length Rrp6p and Rrp6p-ΔC2 lacking its entire C-terminal domain. (B-J) Scattered/Bar plots revealing the steady-state levels of various low molecular weight ncRNAs estimated from the 2 ng cDNA samples prepared using random hexanucleotide primers (pale yellow histograms) or oligo-dT₃₀ anchor primer (dark yellow histograms) by RT-qPCR from the WT strain (yBD-117), yeast strains carrying either a complete deletion of RRP6 (rrp6-Δ; yBD-129) or a deletion of its C-terminal domain (rrp6-ΔC2: yBD-527). SCR1 (in the case of RT-qPCR assays using random primer) and ACT1 mRNA (in the case of RT-qPCR assays using oligo dT₃₀ primer) were used as the internal loading control. Normalized values of each of the ncRNAs in the WT yeast strain were set to 1. Three independent cDNA preparations (biological replicates, n = 3) were used to determine the levels of various ncRNAs. The statistical significance of difference reflected in the ranges of P values estimated from Student’s two-tailed t-tests for a given pair of test strains for every message are presented with the following symbols, *<0.05, **<0.005, and ***<0.001; N, not significant.