Back to article: Two TonB-dependent outer membrane transporters involved in heme uptake in Anabaena sp. PCC 7120
FIGURE 1: Heme utilization by Anabaena. (A) Anabaena was grown in YBG11 (left), YBG11 without iron (second), YBG11 without iron but with 0.5 µM heme (third) or 10 µM heme (right). 50 ml cultures were inoculated to OD750 nm = 0.02 and previously grown in YBG11 medium. Images were taken seven days after inoculation and representatives of multiple replicas are shown. (B, C) The optical density (B) or the chlorophyll a content (C) of Anabaena grown as in (A) was measured and is represented as average of n≥4 experiments with indicated standard deviation. In (B) and (C) the statistical analysis was performed using ANOVA with ranks indicated with p<0.05.