Back to article: The last two transmembrane helices in the APC-type FurE transporter act as an intramolecular chaperone essential for concentrative ER-exit

FIGURE 6: Truncated FurE versions containing the first 10 TMS are ER-retained and do not produce dominant negative effects when co-expressed with wild type FurE. (A) Growth tests and epifluorescence microscopy of controls, strains expressing truncated FurE versions con-sisting of TMS1-10 or TMS11-12, FurE molecules triple alanine substituted in positions 30–32 (Δ30–32) and strains co-expressing combinations of the above constructs with wild type FurE. In each strain one transporter version is GFP-tagged as noted in the strain name. Scale bar corresponds to 5 μM. (B) Radiolabeled uracil uptake rates, expressed as % of wt FurE rate, of strains co-expressing ER-retained FurE versions and wild-type FurE. For details see Materials and Methods.

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