Back to article: A new role for Holliday junction resolvase Yen1 in processing DNA replication intermediates exposes Dna2 as an accessory replicative helicase

FIGURE 1: Dna2 helicase activity promotes the recovery of stalled DNA replication intermediates.

Dna2 acts as an accessory replicative helicase, suppressing toxic replication intermediates and chromosome non-disjunction after RF arrest. This suggests that the Dna2 helicase activity facilitates fork restart or fork protection (green arrows), promoting full genome replication and/or fork fusion during replication termination. Arrested RFs that escape the attention of Dna2 may collapse, triggering HR-mediated restart. Alternatively, unscheduled fork remodeling may occur with the resulting chicken foot structure constituting a potential source for DNA damage checkpoint signaling (lightning bolts) in Dna2 helicase-defective cells, and a possible obstruction to replication termination. These pathways (orange arrows) lead to chromosome entanglements that are resolved by Yen1 in anaphase, ensuring viable chromosome segregation.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.