Back to article: A versatile plasmid system for reconstitution and analysis of mammalian ubiquitination cascades in yeast


FIGURE 2: Galactose inducible expression of E6-E7. Yeast strain yRA2 transformed with E6-E7 cloned in pRA1 (Flag tag, URA3 marker, 93%-GAL1 promoter), pRA2 (Flag tag, URA3 marker, 31%-GAL1 promoter) and pRA3 (Flag tag, URA3 marker, 15%-GAL1 promoter), was induced for the indicated time points by the addition of 2% galactose. Cells were lysed and equivalent amounts of protein extract were analyzed by SDS-PAGE and anti-Flag immunoblotting. Hexokinase is used as a loading control.

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